GlowCas9: A CRISPR Protein that lights up Gene Editing

GlowCas9: A CRISPR Protein that Lights Up Gene Editing

In December 2025, scientists at the Bose Institute, Kolkata developed GlowCas9, a novel CRISPR protein capable of emitting light while carrying out gene editing.

The innovation introduces a new method to directly visualise genome editing activity in real time.

What Makes GlowCas9 Special?

Feature	Details
Real-time Visualisation	GlowCas9 allows scientists to visualise genome editing activity while it is occurring inside living cells.
Bioluminescent Cas9	It is a bioluminescent version of Cas9 created by fusing Cas9 with a split nano-luciferase enzyme derived from deep-sea shrimp proteins.
Light-emitting Mechanism	When Cas9 folds correctly inside a cell, the enzyme fragments rejoin and emit light resembling a gentle firefly glow.
Scientific Advantage	Enables tracking of CRISPR activity in living cells, tissues, and plant leaves without destroying the samples.

Scientific Importance

Improves monitoring of gene-editing efficiency in real time.
Reduces the need to destroy biological samples during analysis.
Can support advances in medical biotechnology, crop science, and genetic research.
Enhances precision and transparency in CRISPR-based applications.

PT Facts

CRISPR-Cas9: A gene-editing tool adapted from a bacterial defence mechanism.
Cas9: Functions like a molecular scissor that cuts DNA at a targeted location.
Bioluminescence: Light produced naturally by living organisms or biological reactions.
GlowCas9: Helps track gene-editing activity in real time without destroying samples.

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